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MSM: Mineral Salts Medium

MSM Deionized water: 1 liter

Dipotassium hydrogen phosphate 2.27 g

Potassium dihydrogen phosphate 0.95 g

Ammonium sulfate 0.67 g

Metals solution* 2 ml

Agar 17.0 g

pH 7.0 ± 0.2

* After autoclaving bulk medium, add 2ml sterile metals solution (recipe below).

MSM metals solution

Deionized water: 1 liter

Na2EDTA.2H2O: 6.37 g

ZnSO4.7H2O: 1.0 g

CaCl2.2H2O: 0.5 g

FeSO4.7H2O: 2.5 g

NaMoO4.2H2O: 0.1 g

CuSO4.5H2O: 0.1 g

CoCl2.6H2O: 0.2 g

MnSO4.H2O: 0.52 g

MgSO4.7H2O: 60.0 g